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Nuclear factor kappa B NF-kappa B is an inducible transcription factor that positively regulates the expression of proimmune and proinflammatory genes, while glucocorticoids are potent suppressors of immune and inflammatory responses. In transient cotransfection experiments, we demonstrate a dose-dependent, mutual antagonism between NF-kappa Magnesium jaw clenching and GR. Functional dissection of the NF-kappa B p50 and p65 subunits and deletion mutants of GR indicate that the GR antagonism is specific to the p65 all free local chat lines of NF-kappa B heterodimer, whereas multiple domains of GR are essential to repress pmediated girl chat show. Despite its repression of GR transactivation, p65 failed to block the transrepressive GR homologous down-regulation function. We also demonstrate that negative interactions between p65 and GR are not selective for GR, but also occur between NF-kappa B and androgen, progesterone B, and estrogen receptors. However, although each of these talks of the steroid hormone receptor family is repressed by NF-kappa B, only GR effectively inhibits p65 transactivation.

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